型号: | PRE001C-50ul |
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品牌: | PREGENE |
原产地: | 美国 |
类别: | 化工 / 生物化学品 |
标签︰ | 内参抗体 , 标签抗体 , 斑马鱼抗体 |
单价: |
¥600
/ 件
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最少订量: | 1 件 |
即时通讯: | 最后上线︰2014/03/04 |
Flag-Tag Mouse Monoclonal Antibody(2C5) |
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Catalog |
PRE001C |
PRE001F |
Tel:010-57129029, 18610667573 |
Quantity |
50μL |
100μL |
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For research use only. |
Applications |
Species Cross-Reactivity |
Molecular Weight |
Isotype |
WB, IP, IF |
N/A |
N/A |
IgG1 |
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.
Recommended dilutions: WB: 1:5,000 IP: 1:200 IF: 1:2000
Optimal dilutions should be determined by the end user.
Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.
Background:The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
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1ug Flag fusion protein+ Primary antibody dilution at 1:10,000
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IP antibody use:5ug Flag Mouse IgG1 per ml Lysate,WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with Flag-tag fusion protein 3、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4、IP (transfected 293+anti- Flag mAb+ Protein G agarose) 5、IP (transfected 293+Protein G) 6、Recombinant protein (E.coli)
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IF analysis of 293 cells transfected with a Flag-tag protein, using anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag) |
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