Hybond带正电荷尼龙膜0.45um
型号: | 30cmx3m RPN303B |
品牌: | Amersham |
原产地: | 美国 |
类别: | 化工 / 生物化学品 |
标签︰ |
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单价: |
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最少订量: | 1 卷 |
产品描述
Amersham Hybond-N+ 带正电荷尼龙膜 0.45um 30cmx3m RPN303B
带正电荷的尼龙膜与核酸高达600μg/cm2的结合能力。建议使用放射性和非放射性的化学发光法和chemifluorescence检测系统。
Amersham公司Hybond™- N +产品在传统的Southern杂交有着出色的灵敏度。核酸样品可能是固定的,通过紫外线照射或烘烤,虽然紫外线固定是最好的最大的重复性。 Hybond N +是不适合碱印迹和固定。
Amersham Hybond™-N+
- Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm2¾recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
- Recommended for all high density membrane arrays.
Technical Information
Amersham Hybond™-N+ yields excellent sensitivity in conventional Southern blotting. Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation is best for maximum reproducibility. Hybond-N+ is not suitable for Alkali blotting and fixation.
- Multiple reprobing is possible without loss of membrane integrity.
- QC tested in genomic Southern blots using GE Healthcare products.
- Special sizes available on request.
Human genomic DNA (1, 0.5, 0.125, 0.06 and 0.03 µg loadings) digested with HindIII and transferred to Hybond-N+. Hybridizaton with A. N-ras, B. p53, and C.
bcl-2 labeled DNA probes and detected using Gene Images™ random-prime labeling with CDP-
Star™ detection.
Membranes should be stored at room temperature in a clean dry atmosphere away from excessive heat, light, and noxious fumes. To prevent contamination, handle membranes using gloves or blunt-ended forceps. Hybond-N+ is stable for up to 3 years. Membranes should be kept in the bags in which they are received. Performance is consistent when stored under the recommended conditions.
TECHNICAL SPECIFICATIONS |
Pore size |
0.45 µm |
Nucleic acid size required |
> 50 bp |
产品图片
图 1
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